Coding
Parseq-

Part:BBa_K3182010:Design

Designed by: Johan Larsson, Jonatan Baggman   Group: iGEM19_Linkoping_Sweden   (2019-07-13)


Parseq-β


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Cellulose binding domain originates from Clostridium thermocellum. AsPink originates from Anemonia sulcata. The GS-linker with the thrombin site and BamHI is of our own design.


Source

Removed one EcoRI site inside the cellulose binding domain. Also added a BamHI site on the end of the thrombin site to enable the ability to change the fusion protein. BamHI was chosen because of the recognition sequence codes for one glycine and one serine, which is also the end recognition of the thrombin site. These amino acids also have very small influence on the fusion protein.

References